• Institute of Respiratory Disease,Xinqiao Hospital,Third Military Medical University.Chongqing,400037,ChinaCorresponding Author:LEI Han,E-mail:leihan@sina.com;
LEI Han, Email: leihan@sina.com
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Objective  To investigate the mRNA and protein expression of human β-defensin-2 (hBD-2) induced by lipopolysaccharide (LPS),IL-1β and TNF-α in human airway primary epitheliums.Methods  The bronchial primary epitheliums from human were stimulated with LPS,IL-1β and TNF-α respectively and then were harvested for hBD-2 expression detection.The mRNA expression of hBD-2 was detected by RT-PCR,and the protein expression by immunocytochemistry and western blot.Results  There was a small expression of hBD-2 mRNA in human airway primary epitheliums before stimulation.The hBD-2 mRNA expression was significantly increased after 3 hours of LPS,IL-1β and TNF-α stimulation respectively and the expression increasement was in a dose dependent manner.The hBD-2 protein could be detected in cytoplasm after 4 hours of LPS (0.1 μg/mL),IL-1β (1 ng/mL) and TNF-α (10 ng/mL) stimulation.Conclusions  LPS and proinflammatory cytokines can induce the mRNA and protein expression of hBD-2 in a short time.The expression of hBD-2 may play an initial defense role against bacterial invasion.

Citation: LIAO Wei,QIAN GuiSheng,LEI Han. Expression of the hBD-2 induced by LPS and proinflammatory cytokines in human primary epitheliums. Chinese Journal of Respiratory and Critical Care Medicine, 2007, 6(2): 127-130. doi: Copy