In order to solve the problem of the micro flow cell clogging, and to improve the reliability of the flow cytometry system, a new method was proposed for hydrodynamic self-cleaning system. By analyzing the flow cell focus principle, we considered that to obtain stable single cell flow, the stable pressure in the flow chamber must be ensured. Therefore, we established a diagnosis method of clogging by the pressure detecting, and designed a self-cleaning system. Then we built up corresponding experimental systems. Experiments and testing showed that the self-cleaning system could improve the flow and resolve the clogging problem.
Citation：
WANGHaijun, GUOYangkuan, ZHULianqing, MENGXiaochen. Design of Flow Cytometry Self-cleaning System. Journal of Biomedical Engineering, 2016, 33(3): 570-574. doi: 10.7507/1001-5515.20160095
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- 1. 吴晓娜,蒋红兵.流式细胞术的工作原理及其临床应用[J].中国医疗设备,2011,26(3):91-93.
- 2. LEE H C, HOU H H, YANG R J, et al. Microflow cytometer incorporating sequential micro-weir structure for three-dimensional focusing[J]. Microfluid Nanofluidics, 2011, 11(4):469-478.
- 3. MILLER S J, JOSHI B P, FENG Ying, et al. In vivo fluorescence-based endoscopic detection of colon dysplasia in the mouse using a novel peptide probe[J]. PLoS One, 2011, 6(3):e17384.
- 4. 赵书涛,武晓东,王策,等.流式细胞仪的原理、应用及最新进展[J].现代生物医学进展,2011,11(22):4378-4381.
- 5. 滕锦,申涛.流动室组件、流动室组件排堵方法及样本分析仪:中国,CN102564919[P].2012-07-11.
- 6. 张兴华,祝捷,翟留伟.血液细胞分析设备中自动排堵方法及系统:中国,CN1556447[P].2013-06-05.
- 7. 张宇.散射成像式流式细胞仪液流系统设计与探究[D].天津:天津大学,2012.
- 8. WATSON D A, GASKILL D F, BROWN L O, et al. Spectral measurements of large particles by flow cytometry[J]. Cytometry A, 2009, 75(5):460-464.
- 9. 杜耀华,陈锋,程智,等.三维液力聚焦的计算设计及其在血细胞分析仪中的应用[J].军事医学,2013,37(8):561-565.
- 10. 马玉婷,严心涛,陈忠祥,等.流式细胞仪液流聚焦系统仿真分析与设计[J].分析仪器,2014,(1):17-22.
- 11. SHAPIRO H M. Practical flow cytometry[M]. 4th ed. New York:John Wiley and Sons Ltd, 2003:166-170.
- 12. 于虎,祝连庆,郭阳宽,等.流式细胞仪流动室流场特性仿真[J].计算机仿真,2015,32(8):230-234.
