Modification of calcium sulfate bone cement by gentamicin and oxygen-carboxymethylated chitosan_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

YUAN Ning ^1,2 ,  LIU Yunde ² , LI Xue ² , ZHANG Lianxiang ¹ , HOU Min ¹ , LI Yinxia ³ , LI Zhaoyang ³

1. Department of Laboratory Medicine, Tianjin Chest Hospital, Tianjin, 300222, P.R.China;
2. College of Laboratory Medicine, Tianjin Medical University, Tianjin, 300203, P.R.China;
3. School of Materials Science& Engineering, Tianjin University, Tianjin, 300350, P.R.China;

Corresponding author：

LIU Yunde, Email: yundeliu@126.com

Keywords：

Calcium sulfate cement; oxygen-carboxymethylated chitosan; gentamicin; antibacterial property

DOI：

10.7507/1002-1892.201604048

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Objective To extend its application in the field of bone repair by adding oxygen-carboxymethylated chitosan (O-CMC) and gentamicin for modification of the calcium sulfate cement (CSC). Methods The O-CMC/CSC was prepared by adding O-CMC with different concentrations (0.1wt%, 0.3wt%, 0.5wt%, 0.7wt%, and 1.0wt%) in the CSC liquid phase. The effect of O-CMC on the CSC was evaluated by testing the injectability, compressive strength, degradation rate, pH value, cytotoxicity and osteogenesis. After the optimal concentration of O-CMC was determined, gentamicin with different concentrations (0.5wt%, 1.5wt%, and 2.5wt%) was added in the O-CMC/CSC, and then the compressive strength and antibacterial properties were investigated. Results After adding O-CMC in the CSC liquid phase, the injection time of O-CMC/CSC was increased to more than 5 minutes; it significantly prolonged with increased concentration of O-CMC (P<0.05). The compressive strength of the modified bone cement was in the range of 11-18 MPa and it was the highest when the concentration of O-CMC was 0.5wt% (P<0.05). The degradation rate of O-CMC/CSC was not influenced obviously by O-CMC (P>0.05). The pH value was in the range of 7.2-7.4 and Ca²⁺ concentration was in the range of 6-8 mmol/L.In vitro mineralization experiment indicated that the induced mineralization ability of O-CMC/CSC was much higher than that of pure CSC. The 0.5wt% O-CMC/CSC had the best performance; the compressive strength of the composite bone cement was above 5 MPa after gentamicin was added, which had antibacterial effect. Conclusion O-CMC is able to effectively improve the injection, compressive strength, and osteogenic activity of CSC; in addition, antibacterial properties is obtained in the CSC after adding gentamicin.

Citation： YUAN Ning, LIU Yunde, LI Xue, ZHANG Lianxiang, HOU Min, LI Yinxia, LI Zhaoyang. Modification of calcium sulfate bone cement by gentamicin and oxygen-carboxymethylated chitosan. Chinese Journal of Reparative and Reconstructive Surgery, 2017, 31(3): 306-312. doi: 10.7507/1002-1892.201604048 Copy

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14.	Kameda T, Mano H, Yamada Y,et al. Calcium-sensing receptor in mature osteoclasts, which are bone resorbing cells. Biochem Biophys Res Commun, 1998, 245(2): 419-422.
15.	吕晓迎, Kappert HF. 牙科材料细胞毒性评定的新方法 (MTT 试验). 中华口腔医学杂志, 1995, 30(6): 377-379.
16.	郜成莹, 叶建东. 添加剂和载药方法对庆大霉素/磷酸钙骨水泥载药体系性能及药物体外释放的影响. 硅酸盐通报, 2008, 27(2): 225-229.

1. Temenoff JS, Mikos AG. Injectable biodegradable materials for orthopedic tissue engineering. Biomaterials, 2000, 21(23): 2405-2412.
2. Peltier LF, Bickel EY, Lillo R,et al. The use of plaster of paris to fill defects in bone. Ann Surg, 1957, 146(1): 61-69.
3. Robinson D, Alk D, Sanbank J,et al. Inflammatory reactions associated with a calcium sulfate bone substitute. Ann Transplant, 1999, 4(3-4): 91-97.
4. Chen J, Gao J, Yin H,et al. Size-controlled preparation of alpha-calcium sulphate hemihydrate starting from calcium sulphate dihydrate in the presence of modifiers and the dissolution rate in simulated body fluid. Mater Sci Eng C Mater Biol Appl, 2013, 33(6): 3256-3262.
5. Cai Z, Zhang T, Di L,et al. Morphological and histological analysis on thein vivo degradation of poly (propylene fumarate)/(calcium sulfate/β-tricalcium phosphate). Biomed Microdevices, 2011, 13(4): 623-631.
6. Vorndran E, Geffers M, Ewald A,et al. Ready-to-use injectable calcium phosphate bone cement paste as drug carrier. Acta Biomater, 2013, 9(12): 9558-9567.
7. 宋阳, 宋广群. 鼻通贴细胞毒性测定方法的确定. 中国医药指南, 2013, 11(24): 66-67.
8. Orellana BR, Thomas MV, Dziubla TD,et al. Bioerodible calcium sulfate/poly(β-amino ester) hydrogel composites. J Mech Behav Biomed Mater, 2013, 26: 43-53.
9. Chang KC, Chang CC, Chen WT,et al. Development of calcium phosphate/sulfate biphasic cement for vital pulp therapy. Dent Mater, 2014, 30(12): e362-e370.
10. Kokubo T, Takadama H. How useful is SBF in predictingin vivo bone bioactivity? Biomaterials, 2006, 27(15): 2907-2915.
11. Athanasiou KA, Zhu C, Lanctot DR,et al. Fundamentals of biomechanics in tissue engineering of bone. Tissue Eng, 2000, 6(4): 361-381.
12. Yaszemski MJ, Payne RG, Hayes WC,et al. Evolution of bone transplantation: molecular, cellular and tissue strategies to engineer human bone. Biomaterials, 1996, 17(2): 175-185.
13. 郜成莹, 叶建东. 磷酸钙骨水泥负载庆大霉素的制备与性能. 材料导报, 2008, 22(3): 151-154.
14. Kameda T, Mano H, Yamada Y,et al. Calcium-sensing receptor in mature osteoclasts, which are bone resorbing cells. Biochem Biophys Res Commun, 1998, 245(2): 419-422.
15. 吕晓迎, Kappert HF. 牙科材料细胞毒性评定的新方法 (MTT 试验). 中华口腔医学杂志, 1995, 30(6): 377-379.
16. 郜成莹, 叶建东. 添加剂和载药方法对庆大霉素/磷酸钙骨水泥载药体系性能及药物体外释放的影响. 硅酸盐通报, 2008, 27(2): 225-229.

Chinese Journal of Reparative and Reconstructive Surgery

Modification of calcium sulfate bone cement by gentamicin and oxygen-carboxymethylated chitosan

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