A model of transplanting neonatal cardiomycytes into the wall of rat inferior vena cava_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

ZHOU Xingang ¹ , NI Lixin ² , CHI Cao ² ,  LIU Hongyu ² , YAO Ye ²

1. Department of Cardiovascular Medicine, Harbin Medical University, Harbin, 150001, P.R.China;
2. Department of Cardiovascular Surgery, Harbin Medical University, Harbin, 150001, P.R.China;

Corresponding author：

LIU Hongyu, Email: hyliu1963@163.com

Keywords：

Neonatal rat; cardiomyocyte; cell transplantation; the wall of inferior vena cava; spontaneously beating

DOI：

10.7507/1007-4848.201612050

Video：

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Objective To establish a model of transplanting neonatal cardiomycytes into the wall of rat inferior vena cava. Methods Neonatal cardiomyocytes (n=6, 5×10⁶cells each, A group) or medium (n=6, B group) only were transplanted into the wall of inferior vena cava in female Fisher rats. At 21 days after transplantation, the contraction of transplanted cardiomyocytes was assessed and the inferior vena cava was processed for histology. Results Distinct rhythmic beating of the vena cava at the site of cell transplantation before and after the aorties were clamped (at a rate 141± 47 rpm and 88± 44 rpm which was dramaticly lower than aortic beating, with a statistical difference at P value of 0.03). Cardiomyocyte was seen in 6 rats who had neonatal cardiomyocyte transplantation, but not in 6 rats receiving media. Hematoxylin and eosin staining showed viable cardiomyocytes in the wall of the vena cava in 6 rats treated with neonatal cardiomyocytes, but not in 6 rats receiving media. Conclusion This study shows that neonatal cardiomyocytes can survive, mature and spontaneously and rhythmically contract after they are transplanted in the wall of inferior vena cava.

Citation： ZHOU Xingang, NI Lixin, CHI Cao, LIU Hongyu, YAO Ye. A model of transplanting neonatal cardiomycytes into the wall of rat inferior vena cava. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2018, 25(3): 233-236. doi: 10.7507/1007-4848.201612050 Copy

1.	钟婷婷, 侯婧瑛, 郭天柱, 等. 心肌干细胞移植可短期内改善心肌梗死心电生理稳定性和提高室颤阈值. 中国组织工程研究, 2015, 28(19): 4537-4543.
2.	张汉平, 蔡晓庆, 马凌. 脂肪间充质干细胞移植治疗兔急性心肌梗死的实验. 国际心血管病杂志, 2015, 6(42): 407-411.
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4.	陈晓明, 崔丹阳, 张明. 急性心肌梗死合并心衰患者应用干细胞移植的临床观察. 大连医科大学学报, 2014, 36(2): 157-159.
5.	侯江龙, 武忠. 细胞移植治疗心肌梗死的研究进展. 中国胸心血管外科临床杂志, 2015, 22(5): 486-490.
6.	Wendel JS, Ye L, Tao R, et al. Functional effects of a tissue-engineered cardiac patch from human induced pluripotent stem cell-derived cardiomyocytes in a rat infarct model. Stem Cells Transl Med, 2015, 4(11): 1324-1332.
7.	Kloner RA, Dai W, Shi J, et al. Rebuilding the infarcted heart with noncellular material. Regen Med, 2015, 10(6): 683-685.
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9.	Gulati AK. Regeneration pattern of cardiac and skeletal muscle after transplantation into a skeletal muscle bed in rats. Anat Rec, 1995, 242(2): 188-194.
10.	Li RK, Mickle DA, Weisel RD, et al. In vivo survival and function of transplanted rat cardiomyocytes.Circ Res, 1996, 78(2): 283-288.
11.	Dai W, Hale SL, Kloner RA. Implantation of immature neonatal cardiac cells into the wall of the aorta in rats a novel model for studying morphological and functional development of heart cells in an extracardiac environment. Circulation, 2004, 110(3): 324-329.
12.	Dai W, Hale SL, Kloner RA. Development of a spontaneously beating vein by cardiomyocyte transplantation in the wall of the inferior vena cava in a rat: A pilot study.J Vasc Surg, 2007, 45(4): 817-820.
13.	Dai W, Hale SL, Kloner RA. Cardiac cells implanted within the outer aortic wall of rats generate measurable contractile force.Regen Med, 2006, 1(1): 119-124.
14.	Biermann D, Eder A, Arndt F, et al.Towards a tissue-engineered contractile Fontan-conduit: the fate of cardiac myocytes in the subpulmonary circulation. PLoS One, 2016, 11(11): e0166963.

1. 钟婷婷, 侯婧瑛, 郭天柱, 等. 心肌干细胞移植可短期内改善心肌梗死心电生理稳定性和提高室颤阈值. 中国组织工程研究, 2015, 28(19): 4537-4543.
2. 张汉平, 蔡晓庆, 马凌. 脂肪间充质干细胞移植治疗兔急性心肌梗死的实验. 国际心血管病杂志, 2015, 6(42): 407-411.
3. 王巍, 李肖甫, 李中健. 不同孕周人脐带间充质干细胞移植改善心肌梗死模型心脏功能的比较. 中国组织工程研究, 2016, 20(6): 799-806.
4. 陈晓明, 崔丹阳, 张明. 急性心肌梗死合并心衰患者应用干细胞移植的临床观察. 大连医科大学学报, 2014, 36(2): 157-159.
5. 侯江龙, 武忠. 细胞移植治疗心肌梗死的研究进展. 中国胸心血管外科临床杂志, 2015, 22(5): 486-490.
6. Wendel JS, Ye L, Tao R, et al. Functional effects of a tissue-engineered cardiac patch from human induced pluripotent stem cell-derived cardiomyocytes in a rat infarct model. Stem Cells Transl Med, 2015, 4(11): 1324-1332.
7. Kloner RA, Dai W, Shi J, et al. Rebuilding the infarcted heart with noncellular material. Regen Med, 2015, 10(6): 683-685.
8. Khan M, Nickoloff E, Abramova T, et al. Embryonic stem cell-derived exosomes promote endogenous repair mechanisms and enhance cardiac function following myocardial infarction. Circ Res, 2015, 117(1): 52-64.
9. Gulati AK. Regeneration pattern of cardiac and skeletal muscle after transplantation into a skeletal muscle bed in rats. Anat Rec, 1995, 242(2): 188-194.
10. Li RK, Mickle DA, Weisel RD, et al. In vivo survival and function of transplanted rat cardiomyocytes.Circ Res, 1996, 78(2): 283-288.
11. Dai W, Hale SL, Kloner RA. Implantation of immature neonatal cardiac cells into the wall of the aorta in rats a novel model for studying morphological and functional development of heart cells in an extracardiac environment. Circulation, 2004, 110(3): 324-329.
12. Dai W, Hale SL, Kloner RA. Development of a spontaneously beating vein by cardiomyocyte transplantation in the wall of the inferior vena cava in a rat: A pilot study.J Vasc Surg, 2007, 45(4): 817-820.
13. Dai W, Hale SL, Kloner RA. Cardiac cells implanted within the outer aortic wall of rats generate measurable contractile force.Regen Med, 2006, 1(1): 119-124.
14. Biermann D, Eder A, Arndt F, et al.Towards a tissue-engineered contractile Fontan-conduit: the fate of cardiac myocytes in the subpulmonary circulation. PLoS One, 2016, 11(11): e0166963.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

A model of transplanting neonatal cardiomycytes into the wall of rat inferior vena cava

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