Objective To investigate the role of leptin receptor gene Gln223Arg polymorphism in pathogenesis of asthma.
Methods One hundred and eighty-five asthmatic outpatients and inpatients in the Qingdao Municipal Hospital between June 2009 and May 2012 were recruited in the study.Two hundred and seven healthy volunteers were recruited as control.Peripheral blood was sampled from all subjects for measuring serum leptin level by ELISA,and analyzing leptin receptor gene Gln223Arg genotypes by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) in white blood cells.
Results There was significant difference in frequency distribution of leptin receptor gene Gln223Arg genotype between the asthma group and the health group (χ2=6.173,P=0.013,OR=1.697,95%CI 1.115-2.585).The GG genotype was associated with a 1.895-fold increased risk for asthma than the GA+AA genotype (χ2=7.283,P=0.007,OR=1.895,95%CI 1.187-3.024).The serum leptin level of the GG genotype group was significantly higher than that in the GA+AA genotype group[(2.56±1.47) ng/mL vs.(2.16±1.66) ng/mL].
Conclusion Leptin receptor gene Gln223Arg polymorphism is correlated with asthma, and the G allele might be the genetic factor that contributes to individual susceptibility for asthma by causing high serum leptin level.
Citation：
GaoJunjie, ZhangWeizhong, JiXia, LiYunxiao. Role of Polymorphism of Leptin Receptor Gene Gln223Arg in Pathogenesis of Asthma. Chinese Journal of Respiratory and Critical Care Medicine, 2014, 13(6): 584-587. doi: 10.7507/1671-6205.2014142
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李锋,张旻,周新.氧化应激与慢性气道疾病.中国呼吸与危重监护杂志,2012,11:97-100.
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Sood A,Ford ES,Camargo CA Jr.Association between leptin and asthma in adults.Thorax,2006,61:300-305.
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Shore SA,Schwartzman IN,Mellema MS,et al.Effect of leptin on allergic airway responses in mice.J Allergy Clin Immunol,2005,115:103-109.
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Mancuso P,Huffnagle GB,Olszewski MA,et al.Leptin corrects host defense defects after acute starvation in murine pneumococcal pneumonia.Am J Respri Crit Care Med,2006,173:212-218.
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Lord GM,Matarese G,Howard JK,et al.Leptin modulates the T-cell immune response and reverses starvation-induced immunosuppression.Nature,1998,394:897-901.
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| 6. |
Chagnon YC,Wilmore JK,Borecki IB,et al.Association between the leptin receptor gene and adiposity in middle-aged Caucasian males from the HERITAGE Family Study.J Clin Endocrinol Metab,2000,85:29-34.
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| 7. |
Chagnon YC,Chung WK,Perusse L,et al.Linkages and associations between the leptin receptor(LEPR) gene human body composition in the Quebec Family Study.Int J Obes,1999,23:278-286.
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| 8. |
Yiannakouris N,Yannakoulia M,Melistas L,et al.The Q223R polymorphism of the leptin receptor gene is significantly associated with obesity and predicts a small percentage of body weight and body composition variability.J Clin Endocrinol Metab,2001,86:4434-4439.
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- 1. 李锋,张旻,周新.氧化应激与慢性气道疾病.中国呼吸与危重监护杂志,2012,11:97-100.
- 2. Sood A,Ford ES,Camargo CA Jr.Association between leptin and asthma in adults.Thorax,2006,61:300-305.
- 3. Shore SA,Schwartzman IN,Mellema MS,et al.Effect of leptin on allergic airway responses in mice.J Allergy Clin Immunol,2005,115:103-109.
- 4. Mancuso P,Huffnagle GB,Olszewski MA,et al.Leptin corrects host defense defects after acute starvation in murine pneumococcal pneumonia.Am J Respri Crit Care Med,2006,173:212-218.
- 5. Lord GM,Matarese G,Howard JK,et al.Leptin modulates the T-cell immune response and reverses starvation-induced immunosuppression.Nature,1998,394:897-901.
- 6. Chagnon YC,Wilmore JK,Borecki IB,et al.Association between the leptin receptor gene and adiposity in middle-aged Caucasian males from the HERITAGE Family Study.J Clin Endocrinol Metab,2000,85:29-34.
- 7. Chagnon YC,Chung WK,Perusse L,et al.Linkages and associations between the leptin receptor(LEPR) gene human body composition in the Quebec Family Study.Int J Obes,1999,23:278-286.
- 8. Yiannakouris N,Yannakoulia M,Melistas L,et al.The Q223R polymorphism of the leptin receptor gene is significantly associated with obesity and predicts a small percentage of body weight and body composition variability.J Clin Endocrinol Metab,2001,86:4434-4439.
