Protection of resveratrol on seawater-drowning-induced lung injury in rats_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

HUANG Yansheng , WEN Wen ,  LAI Guoxiang , PAN Jianguang , SU Shizhen

Department of Respiratory and Critical Care Medicine, No. 900 Hospital of The Chinese People's Liberation Army Joint Logistic Support Force, Fuzhou, Fujian 350000, P. R. China;

Corresponding author：

LAI Guoxiang, Email: laiguoxiang2007@163.com

Keywords：

Pulmonary edema; Seawater drowning; Resveratrol; Apoptosis

DOI：

10.7507/1671-6205.202012019

Video：

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Objective To improve the seawater-drowning-induced lung injury model in rats, and investigate the protective effect of resveratrol against seawater-drowning-induced lung injury and its mechanism.Methods A total of 112 SD healthy rats were randomly assigned into 5 groups: a control group (Group C, n=8), a seawater drowning group (Group S, n=32), a resveratrol prophylactic treatment group (Group S+R, n=32), a resveratrol group (Group R, n=8), and an endotracheal intubation group (Group E, n=32). A modified endotracheal intubation model was developed, and endotracheal intubation was used instead of tracheotomy. Blood gas analysis was performed on the abdominal aorta at each time point, then the rats were sacrificed to obtain their lungs. Lung wet-to-dry ratio (W/D), malondialdehyde (MDA), superoxide dismutase (SOD), myeloperoxidase (MPO) and cysteinyl aspartate specific proteinase (Caspase-3) were measured by enzyme linked immunosorbent assay. The histological sections of rat lungs were stained with haematoxylin-eosin. Groups S+R and R were pretreated with resveratrol (50 mg/kg) through intragastric administration for 3 days; then models were established and the rats were sacrificed 24 hours after the last intragastric administration.Results After seawater perfusion, arterial oxygen pressure decreased and arterial carbon dioxide pressure increased in blood gas analysis of rats, MDA content increased, MPO and SOD activity decreased, caspase-3 content and W/D ratio increased, as well as lung tissue pathological damage. The resveratrol pretreatment group showed the same change trend, but the damage degree was relatively light.Conclusions Seawater perfusion can induce respiratory failure, pulmonary edema and hemorrhage in rats. Lung tissue apoptosis may occur when seawater submergence causes lung injury. Resveratrol pretreatment can ameliorate hypoxia and pulmonary edema in rats.

Citation： HUANG Yansheng, WEN Wen, LAI Guoxiang, PAN Jianguang, SU Shizhen. Protection of resveratrol on seawater-drowning-induced lung injury in rats. Chinese Journal of Respiratory and Critical Care Medicine, 2021, 20(8): 570-576. doi: 10.7507/1671-6205.202012019 Copy

1.	梁源, 金发光. PS 在海水淹溺肺水肿和海水型呼吸窘迫综合征中的治疗作用. 国际呼吸杂志, 2007, 27(18): 1390-1392.
2.	WHO. Global report on drowning: preventing a leading killer. Geneva: World Health Organization, 2016.
3.	Li CC, Bo LY, Li PC, et al. Losartan, a selective antagonist of AT1 receptor, attenuates seawater inhalation induced lung injury via modulating JAK2/STATs and apoptosis in rat. Pulm Pharmacol Ther, 2017, 45: 69-79.
4.	Liu ZY, Xi RG, Zhang ZR, et al. 4-hydroxyphenylacetic acid attenuated inflammation and edema via suppressing HIF-1α in seawater aspiration-induced lung injury in rats. Int J Mol Sci, 2014, 15(7): 12861-12884.
5.	Ji MH, Tong JH, Tan YH, et al. Erythropoietin pretreatment attenuates seawater aspiration-induced acute lung injury in rats. Inflammation, 2016, 39(1): 447-456.
6.	张喜云. 虎杖的化学成分、药理作用与提取分离. 天津药学, 1999, 11(3): 13.
7.	赵克森. 白藜芦醇的生物学特性和效应. 中国病理生理杂志, 2012, 28(9): 1709-1711, 1717.
8.	Bagchi D. Resveratrol and human health. 1st ed. Los Angeles: Keats Pub, 2000.
9.	Aggarwal BB, Bhardwaj A, Aggarwal RS, et al. Role of resveratrol in prevention and therapy of cancer: preclinical and clinical studies. Anticancer Res, 2004, 24(5A): 2783-2840.
10.	赵克森. 虎杖苷抗休克的机理研究. 微循环学杂志, 2006, 16(2): 1-5.
11.	Zhao KS, Jin CH, Huang XL, et al. The mechanism of Polydatin in shock treatment. Clin Hemorheol Microcirc, 2003, 29(3-4): 211-217.
12.	余丹. 氨溴索联合地塞米松治疗海水淹溺性肺损伤的实验研究[D]. 福建医科大学, 2017.
13.	马李杰, 赵诣林, 刘雪英, 等. 乙酰化白藜芦醇保护海水淹溺型急性肺损伤的机制研究. 中国急救医学, 2013, 33(1): 74-76, 后插4.
14.	李聪聪. 蛋白激酶 SPAK 在海水吸入型肺损伤中的作用及相关机制的研究[D]. 第四军医大学, 2014: 1-59.
15.	Zhang ML, Gao YH, Zhao WG, et al. ACE-2/ANG1-7 ameliorates ER stress-induced apoptosis in seawater aspiration-induced acute lung injury. Am J Physiol Lung Cell Mol Physiol, 2018, 315(6): L1015-L1027.
16.	顾兴, 金发光, 傅恩清, 等. 海水淹溺兔肺泡巨噬细胞 TNF-α mRNA 的动态表达及地塞米松干预作用. 现代生物医学进展, 2007, 7(11): 1631-1634.
17.	Lv HM, Liu QM, Wen ZM, et al. Xanthohumol ameliorates lipopolysaccharide (LPS)-induced acute lung injury via induction of AMPK/GSK3β-Nrf2 signal axis. Redox Biol, 2017, 12(2): 311-324.
18.	姜凯丽, 张锡刚. 活性氧在急性肺损伤/急性呼吸窘迫综合征中的作用. 中华损伤与修复杂志(电子版), 2010, 5(2): 249-253.
19.	马李杰. 乙酰化白藜芦醇对大鼠海水吸入型肺损伤的保护作用研究[D]. 第四军医大学, 2013: 1-60.

1. 梁源, 金发光. PS 在海水淹溺肺水肿和海水型呼吸窘迫综合征中的治疗作用. 国际呼吸杂志, 2007, 27(18): 1390-1392.
2. WHO. Global report on drowning: preventing a leading killer. Geneva: World Health Organization, 2016.
3. Li CC, Bo LY, Li PC, et al. Losartan, a selective antagonist of AT1 receptor, attenuates seawater inhalation induced lung injury via modulating JAK2/STATs and apoptosis in rat. Pulm Pharmacol Ther, 2017, 45: 69-79.
4. Liu ZY, Xi RG, Zhang ZR, et al. 4-hydroxyphenylacetic acid attenuated inflammation and edema via suppressing HIF-1α in seawater aspiration-induced lung injury in rats. Int J Mol Sci, 2014, 15(7): 12861-12884.
5. Ji MH, Tong JH, Tan YH, et al. Erythropoietin pretreatment attenuates seawater aspiration-induced acute lung injury in rats. Inflammation, 2016, 39(1): 447-456.
6. 张喜云. 虎杖的化学成分、药理作用与提取分离. 天津药学, 1999, 11(3): 13.
7. 赵克森. 白藜芦醇的生物学特性和效应. 中国病理生理杂志, 2012, 28(9): 1709-1711, 1717.
8. Bagchi D. Resveratrol and human health. 1st ed. Los Angeles: Keats Pub, 2000.
9. Aggarwal BB, Bhardwaj A, Aggarwal RS, et al. Role of resveratrol in prevention and therapy of cancer: preclinical and clinical studies. Anticancer Res, 2004, 24(5A): 2783-2840.
10. 赵克森. 虎杖苷抗休克的机理研究. 微循环学杂志, 2006, 16(2): 1-5.
11. Zhao KS, Jin CH, Huang XL, et al. The mechanism of Polydatin in shock treatment. Clin Hemorheol Microcirc, 2003, 29(3-4): 211-217.
12. 余丹. 氨溴索联合地塞米松治疗海水淹溺性肺损伤的实验研究[D]. 福建医科大学, 2017.
13. 马李杰, 赵诣林, 刘雪英, 等. 乙酰化白藜芦醇保护海水淹溺型急性肺损伤的机制研究. 中国急救医学, 2013, 33(1): 74-76, 后插4.
14. 李聪聪. 蛋白激酶 SPAK 在海水吸入型肺损伤中的作用及相关机制的研究[D]. 第四军医大学, 2014: 1-59.
15. Zhang ML, Gao YH, Zhao WG, et al. ACE-2/ANG1-7 ameliorates ER stress-induced apoptosis in seawater aspiration-induced acute lung injury. Am J Physiol Lung Cell Mol Physiol, 2018, 315(6): L1015-L1027.
16. 顾兴, 金发光, 傅恩清, 等. 海水淹溺兔肺泡巨噬细胞 TNF-α mRNA 的动态表达及地塞米松干预作用. 现代生物医学进展, 2007, 7(11): 1631-1634.
17. Lv HM, Liu QM, Wen ZM, et al. Xanthohumol ameliorates lipopolysaccharide (LPS)-induced acute lung injury via induction of AMPK/GSK3β-Nrf2 signal axis. Redox Biol, 2017, 12(2): 311-324.
18. 姜凯丽, 张锡刚. 活性氧在急性肺损伤/急性呼吸窘迫综合征中的作用. 中华损伤与修复杂志(电子版), 2010, 5(2): 249-253.
19. 马李杰. 乙酰化白藜芦醇对大鼠海水吸入型肺损伤的保护作用研究[D]. 第四军医大学, 2013: 1-60.

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Protection of resveratrol on seawater-drowning-induced lung injury in rats

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