ObjectiveTo study the apoptotic induction effect of Thapsigargin on estrogen receptor positive human breast cancer cell lines MCF7. MethodsCells were treated with Thapsigargin and 5FU in vitro. The rate of cell apoptosis and distribution of cell cycle were detected on flow cytometry. The cell viability was measured by MTT assay and ultrastructural changes in apoptotic cells were confirmed by transmission electron microscopy.ResultsThapsigargin could increase the rates both of cell apoptosis and growth supression of MCF7 cells induced by 5FU and alter the distribution of cell cycle. Under electron microscope, apoptotic bodies in MCF7 cells considerably increased.ConclusionThapsigargin apparently enhances the effect of apoptotic induction of 5FU on MCF7 cells, it is worthy of being further studied.
Citation：
HAN Jiangtao,YAO Zhenxiang. Research on the Induction of Apoptosis in MCF7 Cells Enhanced by Thapsigargin. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(3): 231-233. doi:
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- 2. Korge P, Weiss JN. Thapsigargin directly induces the mitochondrial permeability transition [Ｊ]. Eur J Biochem， 1999; 265(1)∶273.
- 3. 周光飚， 张广森. 吲哚美辛诱导K562细胞凋亡过程中半胱天冬酶及细胞内游离钙浓度的变化 [Ｊ]. 中华血液学杂志， 2001； 22（5）∶241.
- 4. Shen ZY, Shen WY, Chen MH, et al. Nitric oxide and calcium ions in apoptotic esophageal carcinoma cells induced by arsenite [Ｊ]. World J Gastroenterol, 2002; 8(1)∶40.
- 5. 杨光华主编. 病理学 [M] . 第5版. 北京： 人民卫生出版社， 2001∶84～92.
- 6. 刘大川， 姚榛祥， 李非， 等. 雌激素受体阴性乳癌细胞株中Cyclin D1和Ki67表达与细胞凋亡的关系 [Ｊ]. 中国普外基础与临床杂志， 2003； 10（1）∶86.
- 7. 姚榛祥. 乳腺癌诊治的进展 [Ｊ]. 中国普外基础与临床杂志， 2002； 9（5）∶293.
- 8. Lucas M, Diaz P. Thapsigargininduced calcium entry and apoptotic death of neutrophils are blocked by activation of protein kinase C [Ｊ]. Pharmacology， 2001; 63(3)∶191.
