SD mice were selected for Collin’s solution (4℃) infusion into the portal vein with different pressure to preserve the liver transplants. The following parameters were determined ①liver tissue aderine ribonucleotide including adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), ②cytoplasmic free Ca２＋ in single liver cell (［Ca２＋］i) and ③tissue pathologic ultrastructure change by highperformance liquid chromatography into quantimeter and pathologic examination respectively. The result suggested that with the infusion pressure becoming higher, the liver free Ca２＋(［Ca２＋］i), tissue aderine ribonucleotide, EC and tissue pathologic ultrastructure changed obviously. This result shows ［Ca２＋］i, EC and tissue aderine ribonucleotide might indicate the viability of liver transplant, and using low pressure infusion has benefit effect on liver preservation.
Key wordsCold infusion pressureViability of liver transplantEnergy metabolismLiver cell free Ca２＋
Citation：
Zhang Hao,Wang Xuehao,Zhang Feng.. THE PRESSURE OF INFUSION AFFECTING ON HEPATIC TRANSPLANT. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1999, 6(3): 140-142. doi:
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Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
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- 1. Ursula R, Maren H, Werner L. Energydependent injury to cultured sinusoidal endothelial cells of the rat liver in UW solution. Transplantation, 1993; 55(3)∶469.
- 2. Kamiikke W, Burdelski M, Steinhoff G, et al. Adenine nucleotide methabolism and its relation to organ viability in human liver transplantation. Transplantation, 1988; 45(1)∶138.
- 3. Hidication R, Rose Innes C, Tyler M, et al. Energy charge as an indication of liver viability. Transplantation, 1992; 53(4)∶540.
- 4. Foegh ML, Khirabadi BS, Ramwell PW. Prolongation of rat cardiac allograft survival by Ca2＋ blockers. Transplantation, 1985; 40(2)∶211.
- 5. Kimura J, Nakajima Y, Omura T, et al. Warm ischemia impairs calcium metabolism in porcine grafted liver. Transplant Proc, 1994; 26(4)∶2272.
- 6. Nishikawa Y, Ukida M, Matsuo R. Ca2＋ influx initiates death of hepatocytes injured by activation of complement. Liver, 1994; 14(4)∶200.
