• Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu Sichuan，610041，P. R. China;
Tissue engineered bone; Marrow mesenchymal stem cells; Porous biphasic calcium phosphate scaffold; Rat
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Objective To study the adhesion characteristic in vitrobetween porous biphasic calcium phosphate(BCP) nanocomposite and bone marrow mesenchymal stem cells (MSCs) that have been induced and proliferated. Methods MSCs obtained from SD ratbone marrow were in vitro induced and proliferated. After their osteoblastic phenotype were demonstrated, MSCs were seeded onto prepared porous BCP nanocomposite(experiment group)and common porous hydroxyapatite (control group). Their adhesion situation was analyzed by scanning electron microscope. The initial optimal cell seeding density was investigated between new pattern porous BCP nanocomposite and MSCs by MTT automated colormetric microassay method. Results The differentiation of MSCs to osteoblastic phenotype were demonstrated by the positive staining of mineralized node, alkaline phosphatase (ALP) and collagen typeⅠ, the most appropriate seeding density between them was 2×106／ml. The maximal number which MSCs could adhere to porous BCP nanocomposite was 1.28×107/cm3. Conclusion MSCs can differentiate to osteoblastic phenotype.The MSCs were well adhered to porous BCP nanocomposite.

Citation： WANG Tao,TIAN Weidong,CHEN Xizhe,et al.. EXPERIMENTAL STUDY ON CELL ADHESION CHARACTERISTIC BETWEEN POROUS BIPHASIC CALCIUM PHOSPHATE NANOCOMPOSITE AND BONE MARROW MESENCHYMAL STEM CELLS IN VITRO. Chinese Journal of Reparative and Reconstructive Surgery, 2006, 20(5): 565-568. doi: Copy

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