Objective To observe the effect of RNA interference (RNAi) on HepG2 hepatic cancer cell by small interfering RNA (siRNA).
Methods siRNA targeting vascular endothelial growth factor (VEGF) gene was transfected into HepG2 cells by LipofectimineTM 2000. The VEGF mRNA and protein were respectively detected by real-time quantitive PCR and Western blot, and the concentration of VEGF protein in the cell culture supertant was determined by ELISA at 48 h after culture.
Results The average efficiency of siRNA transfection was (90.4±2.9)% after 6 h cell culture. The expressions of VEGF mRNA and protein in HepG2 cells could be effectively suppressed by siRNA, and the concentration of VEGF protein in the cell culture supertant was also decreased.
Conclusion siRNA can knock down the expression of VEGF gene and decrease the concentration of VEGF protein in HepG2 cells.
Citation：
ZHU Hongbo ,CHEN Zhaoyong,WANG Yong,ZHOU Xiaochun,WANG Jianxin. Study of Inhibition Effects of Small Interfering RNA on VEGF Gene Expression in HepG2 Hepatic Cancer Cell. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2009, 16(8): 633-636. doi:
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| 1. |
Elbashir SM, Martinez J, Patkaniowska A, et al. Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate [J]. EMBO J, 2001; 20(23): 6877-6888.
|
| 2. |
Ferrara N, Gerber HP, LeCouter J. The biology of VEGF and its receptors [J]. Nat Med, 2003; 9(6): 669-676.
|
| 3. |
Fire A, Xu S, Montgomery MK, et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].Nature, 1998; 391(6669): 806-811.
|
| 4. |
Semizarov D, Frost L, Sarthy A, et al. Specificity of short interfering RNA determined through gene expression signatures [J]. Proc Natl Acad Sci USA, 2003; 100(11): 6347-6352.
|
| 5. |
邓伟， 梁力建. 脂质体Tie-2-siRNA表达载体对HepG2细胞裸鼠移植瘤作用的研究 [J]. 中国普外基础与临床杂志， 2007; 14(5): 543-546.
|
| 6. |
Cruz PE, Mueller C, Cossette TL, et al. In vivo post-transcriptional gene silencing of alpha-1 antitrypsin by adeno-associated virus vectors expressing siRNA [J]. Lab Invest, 2007; 87(9): 893-902.
|
| 7. |
Folkman J. Clinical application of research of angiogenesis [J]. N Engl J Med, 1995; 333(26): 1757-1763.
|
| 8. |
Carpenter B, Lin Y, Stoll S, et al. VEGF is crucial for the hepatic vascular development required for lipoprotein uptake [J]. Development, 2005; 132(14)： 3293-3303.
|
| 9. |
Yamaguchi R, Yano H, Iemura A, et al. Expression of vascular endothelial growth factor in human hepatocellular carcinoma [J]. Hepatology, 1998; 28(1): 68-77.
|
| 10. |
Poon RT, Lau C, Yu WC, et al. High serum levels of vascular endothelial growth factor predict poor response to transarterial chemoembolization in hepatocellular carcinoma: a prospective study [J]. Oncol Rep, 2004; 11(5): 1077-1084.
|
| 11. |
解乃昌， 李国威， 王志亮， 等. 抗血管内皮生长因子抗体对实验性肝癌的生长抑制作用 [J]. 中国普外基础与临床杂志， 2002; 9(4): 242-245.
|
- 1. Elbashir SM, Martinez J, Patkaniowska A, et al. Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate [J]. EMBO J, 2001; 20(23): 6877-6888.
- 2. Ferrara N, Gerber HP, LeCouter J. The biology of VEGF and its receptors [J]. Nat Med, 2003; 9(6): 669-676.
- 3. Fire A, Xu S, Montgomery MK, et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].Nature, 1998; 391(6669): 806-811.
- 4. Semizarov D, Frost L, Sarthy A, et al. Specificity of short interfering RNA determined through gene expression signatures [J]. Proc Natl Acad Sci USA, 2003; 100(11): 6347-6352.
- 5. 邓伟， 梁力建. 脂质体Tie-2-siRNA表达载体对HepG2细胞裸鼠移植瘤作用的研究 [J]. 中国普外基础与临床杂志， 2007; 14(5): 543-546.
- 6. Cruz PE, Mueller C, Cossette TL, et al. In vivo post-transcriptional gene silencing of alpha-1 antitrypsin by adeno-associated virus vectors expressing siRNA [J]. Lab Invest, 2007; 87(9): 893-902.
- 7. Folkman J. Clinical application of research of angiogenesis [J]. N Engl J Med, 1995; 333(26): 1757-1763.
- 8. Carpenter B, Lin Y, Stoll S, et al. VEGF is crucial for the hepatic vascular development required for lipoprotein uptake [J]. Development, 2005; 132(14)： 3293-3303.
- 9. Yamaguchi R, Yano H, Iemura A, et al. Expression of vascular endothelial growth factor in human hepatocellular carcinoma [J]. Hepatology, 1998; 28(1): 68-77.
- 10. Poon RT, Lau C, Yu WC, et al. High serum levels of vascular endothelial growth factor predict poor response to transarterial chemoembolization in hepatocellular carcinoma: a prospective study [J]. Oncol Rep, 2004; 11(5): 1077-1084.
- 11. 解乃昌， 李国威， 王志亮， 等. 抗血管内皮生长因子抗体对实验性肝癌的生长抑制作用 [J]. 中国普外基础与临床杂志， 2002; 9(4): 242-245.
