Objective To explore the effects of CO2 pneumoperitoneum on pancreatic function in diabetic rabbits.
Methods Forty-eight rabbits were divided into 4 groups: control group (the group of N0, n=4), the group of T0 (n=4), the group of T10 (n=20), and the group of T15 (n=20). The animal used in the groups of T0, T10 and T15 was diabetic rabbit, and the pressures of pneumoperitoneum of the three groups were 0 mm Hg, 10 mm Hg and 15 mm Hg respectively.The model of diabetic rabbits were made through intrvenous administration of Allxon. Arterial blood samples were collected before the onset of CO2 pneumoperitoneum, 0, 2, 6, 12 hours after deflation for measuring blood glucose, amylase, insulin and C-peptid. Then the rabbits were sacrificed and their pancreases were removed for measuring SOD activity and MDA content.
Results After abdominal deflation, the blood glucose, amylase, insulin, C-peptid, MDA content were significantly increased （P＜0.05), and SOD activity was significantly decreased（P＜0.05). Twelve hours after abdominal deflation, the levels of blood glucose, amylase, insulin, C-peptid, MDA content returned to those before pneumoperitoneum was established in group T10. But, those in group T15 were higher （P＜0.05) than the levels before insufflation. The SOD activities in both group T10 and group T15 twelve hours after abdominal deflation were significantly different （P＜0.05） from those before pneumoperitoneum was established. There were statistically significant differences （P＜0.05) between group T10 and T15 in amylase, C-peptid, MDA content and SOD activity.
Conclusion CO2 pneumoperitoneum has an certain adverse influence on pancreatic function of the diabetic rabbits. The degree of injury is correlated with the pressure of pneumoperitoneum. Pancreatic function may returned to preoperative level soon after abdominal deflation in group T10, but did not return in group T15.
Citation：
ZHU Shaohui,LUO Ding,CHEN Xunru. Effects of CO2 Pneumoperitoneum on Pancreatic Function in Diabetic Rabbits. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(4): 428-431. doi:
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Nagaoka H, Innami R, Watanabe M, et al. Preservation of pancreatic beta cell function with pulsatile cardiopulmonary bypass [Ｊ] . Ann Thorac Surg, 1989; 48(6)∶798.
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Glerup H, Heindorff H, Flyvbjerg A, et al. Elective laparoscopic cholecystectomy nearly abolishes the postoperative hepatic catabolic stress response [Ｊ] . Ann Surg， 1995; 221(3)∶214.
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Myre K, Rostrup M, Eriksen M, et al. Increased spillover of norepinephrine to the portal vein during CO-pneumoperitoneum in pigs [Ｊ] . Acta Anaesthesiol Scand, 2004; 48(4)∶443.
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Gudmundsson FF, Gislason HG, Dicko A, et al. Effects of prolonged increased intra-abdominal pressure on gastrointestinal blood flow in pigs [Ｊ] . Surg Endosc, 2001; 15(8)∶854.
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Schafer M, Sagesser H, Reichen J, et al. Alterations in hemodynamics and hepatic and splanchnic circulation during laparoscopy in rats [Ｊ] . Surg Endosc, 2001; 15(10)∶1197.
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Yavuz Y, Ronning K, Lyng O, et al. Effect of increased intraabdominal pressure on cardiac output and tissue blood flow assessed by color-labeled microspheres in the pig [Ｊ] . Surg Endosc, 2001; 15(2)∶149.
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闫宏宪, 罗丁, 陈训如, 等. CO2气腹对肝硬变大鼠肠黏膜通透性影响的实验研究 [Ｊ] . 中国普外基础与临床杂志, 2005; 12(6)∶548.
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Cohen R, Schiavon CA, Schaffa TD. Avoiding complications in closed pneumoperitoneum (umbilicus lifting) insufflation, retaining good cosmetic results [Ｊ] . Surg Endosc, 1995; 9(5)∶543.
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Bickel A, Arzomanov T, Ivry S, et al. Reversal of adverse hemodynamic effects of pneumoperitoneum by pressure equilibration [Ｊ] . Arch Surg, 2004; 139(12)∶1320.
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Kingsnorth A. et al. Role of cytokines and their inhibitors in acute pancreatitis [Ｊ] . Gut, 1997; 40(1)∶1.
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Schoenberg MH, Buchler M, Younes M, et al. Effect of antioxidant treatment in rats with acute hemorrhagic pancreatitis [Ｊ] . Dig Dis Sci, 1994; 39(5)∶1034.
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| 13. |
Redl H, Gasser H, Schlag G, et al. Involvement of oxygen radicals in shock related cell injury [Ｊ] . Br Med Bull, 1993; 49(3)∶556.
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| 14. |
吴辉, 陈训如, 罗丁, 等. CO2气腹后慢性肾衰大鼠肾脏急性时相期病理学的改变 [Ｊ] . 中国内镜杂志, 2003; 9(1)∶12.
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| 15. |
查勇, 陈训如, 罗丁. CO2气腹对动脉粥样硬化兔颈动脉血流量的影响 [Ｊ] . 中国普外基础与临床杂志, 2005; 12(6) ∶544.
|
- 1. 方厚华主编. 医学试验模型动物 [M] . 第2版. 北京: 军事医学科学出版社, 2002∶55~58.
- 2. Nagaoka H, Innami R, Watanabe M, et al. Preservation of pancreatic beta cell function with pulsatile cardiopulmonary bypass [Ｊ] . Ann Thorac Surg, 1989; 48(6)∶798.
- 3. Glerup H, Heindorff H, Flyvbjerg A, et al. Elective laparoscopic cholecystectomy nearly abolishes the postoperative hepatic catabolic stress response [Ｊ] . Ann Surg， 1995; 221(3)∶214.
- 4. Myre K, Rostrup M, Eriksen M, et al. Increased spillover of norepinephrine to the portal vein during CO-pneumoperitoneum in pigs [Ｊ] . Acta Anaesthesiol Scand, 2004; 48(4)∶443.
- 5. Gudmundsson FF, Gislason HG, Dicko A, et al. Effects of prolonged increased intra-abdominal pressure on gastrointestinal blood flow in pigs [Ｊ] . Surg Endosc, 2001; 15(8)∶854.
- 6. Schafer M, Sagesser H, Reichen J, et al. Alterations in hemodynamics and hepatic and splanchnic circulation during laparoscopy in rats [Ｊ] . Surg Endosc, 2001; 15(10)∶1197.
- 7. Yavuz Y, Ronning K, Lyng O, et al. Effect of increased intraabdominal pressure on cardiac output and tissue blood flow assessed by color-labeled microspheres in the pig [Ｊ] . Surg Endosc, 2001; 15(2)∶149.
- 8. 闫宏宪, 罗丁, 陈训如, 等. CO2气腹对肝硬变大鼠肠黏膜通透性影响的实验研究 [Ｊ] . 中国普外基础与临床杂志, 2005; 12(6)∶548.
- 9. Cohen R, Schiavon CA, Schaffa TD. Avoiding complications in closed pneumoperitoneum (umbilicus lifting) insufflation, retaining good cosmetic results [Ｊ] . Surg Endosc, 1995; 9(5)∶543.
- 10. Bickel A, Arzomanov T, Ivry S, et al. Reversal of adverse hemodynamic effects of pneumoperitoneum by pressure equilibration [Ｊ] . Arch Surg, 2004; 139(12)∶1320.
- 11. Kingsnorth A. et al. Role of cytokines and their inhibitors in acute pancreatitis [Ｊ] . Gut, 1997; 40(1)∶1.
- 12. Schoenberg MH, Buchler M, Younes M, et al. Effect of antioxidant treatment in rats with acute hemorrhagic pancreatitis [Ｊ] . Dig Dis Sci, 1994; 39(5)∶1034.
- 13. Redl H, Gasser H, Schlag G, et al. Involvement of oxygen radicals in shock related cell injury [Ｊ] . Br Med Bull, 1993; 49(3)∶556.
- 14. 吴辉, 陈训如, 罗丁, 等. CO2气腹后慢性肾衰大鼠肾脏急性时相期病理学的改变 [Ｊ] . 中国内镜杂志, 2003; 9(1)∶12.
- 15. 查勇, 陈训如, 罗丁. CO2气腹对动脉粥样硬化兔颈动脉血流量的影响 [Ｊ] . 中国普外基础与临床杂志, 2005; 12(6) ∶544.
