Objective To investigate the expression of multigenes mediated by adenovirus in liver cancer cells and the effects on growth of cells transducted with multigenes.
Methods By construction of recombinant adenovirus containing human p53, B7-1, GM-CSF, and IL-2 genes (Ad-multigenes), the expression level of target genes in three human hepatocellular carcinoma cell lines and a human hepatocellular cell line L02 was detected using ELISA, immunohistochemistry and FACS assay and the change of growth of these cells and the tumor cell apoptosis were observed.
Results The human hepatic cells and liver cancer cells were all sensitive to adenovirus infection. At a MOI of 50 PFU/cell, among the cells examined nearly 90% were positive expression and except IL-2, other three genes were expressed with high efficiency. The growth of Ad-multigenes-transduced liver cancer cell lines was inhibited and apoptosis was induced, but the growth of Ad-multigenes-transduced normal hepatic cell line L02 did not change. Conclusion These results indicate that the adenovirus is an efficient vector for gene transfer into human liver cancer cells. These liver cancer cell lines transduced with multigenes constructed on one recombinant adenoviral vector can highly express target genes and their growth was inhibited, and apoptosis appeared.
Citation：
WANG Zhengx,HE Zhenping,WU Zuze.. EFFECTS OF ADENOVIRAL VECTOR-MEDIATED MULTIGENES ON LIVER CANCER CELLS GROWTH. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2000, 7(6): 357-359下转361. doi:
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- 2. 王征旭， 何振平， 吴祖泽. 肿瘤基因工程瘤苗研究进展 〔Ｊ〕. 国外医学肿瘤学分册， 1998； 25(1)∶1.
- 3. 王征旭， 何振平， 吴祖泽等. 腺病毒介导多基因在胆管癌细胞中的表达 〔Ｊ〕. 第三军医大学学报， 1999； 21(10)∶742.
- 4. 王征旭， 何振平， 吴祖泽等. 腺病毒介导多基因对胆管癌细胞凋亡的诱导和肝细胞毒性损害 〔Ｊ〕. 第三军医大学学报， 1999； 21(11)∶780.
- 5. Tatsumi T, Takehara T, Katayama K, et al. Expression of costimulatory molecules B71 (CD80) and B72 (CD86) on human hepatocellular carcinoma 〔Ｊ〕. Hepatology, 1997; 35(4)∶1108.
- 6. Zhang WW, Fang X, Mazur W, et al. Highefficiency gene transfer and highlevel expression of wildtype p53 in human lung cancer cells mediated by recombinant adenovirus 〔Ｊ〕. Cancer Gene Ther, 1994; 1(1)∶5.
- 7. Nielsen LL, Maneval DC. p53 tumor suppressor gene therapy for cancer 〔Ｊ〕. Cancer Gene Ther, 1998; 5(1)∶52.
- 8. Anderson SC, Johnson DE, Harris MP, et al. p53 gene therapy in a rat model of hepatocellular carcinoma: intraarterial delivery of a recombinant adenovirus 〔Ｊ〕. Clin Cancer Res, 1998; 4(6)∶1649.
