Objective To review the current concepts of gene therapy approachesmediated by adenovirus vectors for bone trauma and bone disease. Methods The recent literature concerned gene therapy mediated by adenovirus vectors was reviewed, which provides new insights into the treatments of bone trauma and bone disease. Results Adenovirus vectors was efficient, achieved high expression after transduction, and could transfer genes to both replicating and nonreplicating cells, such as osteoblasts, osteoclasts, fibroblasts, chondrocytes, bone marrow stromal cells, etc. Gene therapy mediated by adenovirus vectors achieved affirmative results in enhancing bone union and in curing bone diseases, such as osteoporosis and rheumatoid arthritis. Conclusion Gene therapy mediatedby adenovirus offers an exciting avenue for treatment of bone trauma and bone diseases.
目的 总结躯干及肢体大血管损伤救治的经验体会。 方法 对37例躯干及四肢大血管损伤病例资料进行回顾性分析，总结其院前急救、手术方法和围手术期处理经验。结果 全组病例中除2例因失血性休克死亡外，其余病例均痊愈，无血管损伤术后并发症。结论 加强基层医院医生在躯干及四肢大血管创伤救治技术上的培训并得以提高，有利于降低大血管损伤的死亡率和致残率。
Objective To evaluate the effectiveness of Ilizarov external fixation and ankle arthrodesis in the treatment of late traumatic ankle arthritis. Methods Between June 2013 and June 2015, 27 patients with late traumatic ankle arthritis were treated with Ilizarov external fixation technique. There were 16 males and 11 females with an age of 27-69 years (mean, 45.7 years). Sixteen cases were on the left side, 11 on the right side. All the patients suffered from traumatic ankle fractures or ligament damages caused by initial traumas. After 6 months of standard conservative treatment, the results was invalid and all patients had ankle joint pain and movement disorders. The disease duration was 3-39 years (mean, 11.5 years). According to Takakura ankle arthritis staging, there were 16 cases in stage 3 and 11 cases in stage 4. The tibial-talar angle before operation was (102.55±4.02) ° measured on conventional double-feet loading anteroposterior and lateral X-ray films. The American Orthopaedic Foot and Ankle Society (AOFAS) ankle and foot joint score was 45.72±6.45, and the visual analogue scale (VAS) score was 8.61±1.96. Results All the patients were followed up 15-42 months (mean, 28.1 months). All ankles achieved bony fusion, the clinical healing time was 12.9 weeks on average (range, 11-18 weeks). No persistent bleeding in the incisions and needle tract occurred during the follow-up. There were 4 cases of mild needle infection, 2 cases of anterior dislocation of talus, and 3 cases with different degree of limited activity. No traumatic bone defect, bone disconnection, and false joint formation was observed. At 12 months after operation, the AOFAS ankle and foot joint score, VAS score, and tibial-talar angle were 80.53±9.14, 2.77±0.82, and (94.36±2.48)°, respectively, which were significantly improved when compared with preoperative ones (t=16.17, P=0.00; t=14.28, P=0.00; t=9.01, P=0.00). The effectivenss was excellent in 9 cases, good in 13 cases, and fair in 5 cases, with an excellent and good rate of 81.5%. Conclusion Satisfactory effectiveness can be obtained through Ilizarov external fixation and ankle arthrodesis in the treatment of traumatic ankle arthritis, showing certain application prospect, while long-term effectiveness should be comfirmed by large sample randomized controlled trials.
Fifteen rabbits were given a second degree scald on the back, then a large amount of fluid were given rapidly. In this way an acute diluted hyponatremia and a brain edema model were produced. In one group, the magnetotherapy was instituted immediately following the acut injury, while in the other group, it was started after the brain edema being developed. After giving a large quantity of fluid, the Na~+, CI~-, and K~+ concentrations in the blood were decreased and the quantity of water in the brain tissue was increased evidentely. The phenomena of neuron edema and widening of the perivascular space were also found. It was proved that the model of experimental brain edema could be produced on animals and the treatment and the prevention for brain edema could be obtained to advantage to certaiin degree. Also,it was better to use the magnetotherapy in brain edema as early as possidle.
Abstract For the purpose of studying the effects on wound healing of three new bioelectret composites (BC) which are composed ofcollagen polyvinyl alcohol and epidermal growth factor (EGF), 30 rabbits were divided into 5 groups. Three wounds in round shape (1.8 cm in diameter) were made in each side of the back of the rabbits. The wounds of 1～3 groups were treated by one of the three BC respectively, group 4 treated by SDAg, and group 5 were treated by Normal saline as control. From observation of the growth of the granulation tissue, the reepithelization and the pathological assessment, it was shown that the quality of wound healing in all BC treated wounds was better than that in SDAg or the control. It indicated that the BC benefited the wound healing, and this might also be due to its bioelectric effects and the direct effects from growth factor.
目的 探讨单纯肠系膜损伤的诊断手段及治疗方法。方法 回顾性分析笔者所在医院2009年1月至2011年12月期间收治的12例单纯肠系膜损伤患者的临床资料。结果 12例患者均为腹部闭合性损伤，其中坠落伤2例，车祸伤7例，挤压伤2例，踢打伤1例。均行手术治疗，其中9例系膜破裂出血患者中，3例行系膜血管结扎、系膜修补术，6例行小肠部分切除、系膜修补术；另3例系膜血肿患者均行小肠部分切除术。术后发生切口感染2例，炎性肠梗阻1例，均经对症治疗后好转。其余9例均治愈出院，无并发症发生；无死亡病例。结论 对系膜破裂出血型肠系膜损伤患者应早期行手术治疗，系膜血肿型患者应密切观察病情变化以及做必要的辅助检查，以防止误诊及漏诊。