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ROLE OF STEM CELL NICHES IN MAINTAINING CARDIAC STEM CELLS HOMEOSTASIS

ObjectiveTo review the role of stem cell niches in maintaining cardiac stem cells homeostasis, and to foresee its prospects. MethodsThe literature on cardiac stem cells niches was extensively reviewed. The roles of stem cell niches components, extracellular matrix, and secretory factors in maintaining cardiac stem cell homeostasis were analysed and reviewed. ResultsLots of experiments reveal that stem cell niches are able to delay the aging of cardiac stem cells, protect from external damage, keep stem properties, and improve the purity and quantity. However, the mechanism is not fully understood. ConclusionThe stem cell niches have a very bright application prospect in homeostasis, purification, and amplification for the cardiac stem cells, and it needs further study.

RESEARCH PROGRESS OF MYOCARDIAL TISSUE ENGINEERING SCAFFOLD MATERIALS

Objective To review the current status and problems in the developing scaffolds for the myocardial tissue engineering appl ication. Methods The l iterature concerning the myocardial tissue engineering scaffold in recent years was reviewed extensively and summarized. Results As one of three elements for tissue engineering, a proper scafold is veryimportant for the prol iferation and differentiation of the seeding cells. The naturally derived and synthetic extracellular matrix (ECM) materials aim to closely resemble the in vivo microenvironment by acting as an active component of the developing tissue construct in myocardial tissue engineering. With the advent and continuous refinement of cell removal techniques, a new class of native ECM has emerged with some striking advantages. Conclusion Through using the principle of composite scaffold, computers and other high-technology nano-polymer technology, surface modification of traditional biological materials in myocardial tissue engineering are expected to provide ideal myocardial scaffolds.

食管中段鳞癌合并胃神经鞘瘤一例

心脏刀刺伤15例

肺曲菌病八例

同种异体带瓣管道在复杂先天性心脏病中的应用

摘要： 目的 探讨同种异体带瓣管道在复杂先天性心脏病中的临床应用价值。 方法 2004年10月至2007年5月济宁医学院附属医院共收治7例复杂先天性心脏病患者，男4例，女3例；年龄5~18岁，平均年龄13岁；体重13~57 kg，平均体重35 kg。所有患者均行同种异体主动脉带瓣管道右心室流出道重建术，同期行动脉导管结扎加法洛四联症(TOF)根治术2例，动脉导管结扎、TOF根治、主动脉瓣成形术1例，TOF根治、左右肺动脉吻合术1例，右心室双出口根治术2例，矫正型大动脉转位、室间隔缺损修补术1例。 结果 手术死亡1例（14.29%），因手术后发生严重低心排血量综合征，术中停机困难死亡。1例患者术后7d出现阵发性室上性心动过速，给予甲基强的松龙和胺碘酮口服后好转。随访6例，随访时间6个月~4年，随访期间有1例术后6个月因持续发热经抗感染治疗无效，最终死于脑出血。5例生存患者紫绀消失，心功能Ⅰ~Ⅱ级，生长发育良好，已恢复正常学习。 结论 同种异体带瓣管道在一些复杂先天性心脏病治疗中有良好的临床应用价值。

22例心脏肿瘤的诊断与治疗

RESEARCH PROGRESS IN CARDIAC BIOLOGICAL PACEMAKER CREATED BY CELL TRANSPLANTATION

Objective To review the current status and problems in developing cardiac biological pacemaker(CBP) by cell transplantation. Methods The l iterature over the past decade concerning CBP constructed through celltransplantation was reviewed and summarized. Results Experiments in vivo testified that the cell transplantation was feasible for CBP construction, and the transplantation of sinus atrial node cell and stem cell was still the predominant method for constructing CBP. However, such problems as difficult ampl ification of transferred cardio muscle cell, low success rate of CBP construction as well as unstable function of CBP make it lag behind the tremendous cl inical demands. The gene transfection technology might be one of the approaches to resolve these issues. Conclusion As one feasible method for CBP construction, the cell transplantation has a bright future in the cl inical appl ication and is worthy of further study.

肺结核球103例

小肠黏膜下层-骨髓间充质干细胞移植-治疗陈旧性心肌梗死

摘要: 目的 探讨自体骨髓间充质干细胞(MSCs)小肠黏膜下层(SIS)支架复合物移植于陈旧性心肌梗死区域后细胞的存活、支架的降解情况及其对心功能的影响。 方法 将16只黑山羊按随机数字表法分为两组，每组8只。实验组：建立心肌梗死模型，抽取自体骨髓,经体外分离MSCs,进行培养、传代、BrdU标记、与SIS支架复合，并于心肌梗死6周时将MSCs-SIS复合物补片移植至陈旧性心肌梗死区；对照组：建立心肌梗死模型。于移植后6周行超声心动图、HE染色及免疫组织化学检测。 结果 MSCs-SIS植入心肌梗死区2周时在补片区可见大量炎性细胞浸润；6周时淋巴细胞浸润消失，SIS部分降解，移植区见大量移植细胞存活。MSCs-SIS植入体内后6周时，实验组每搏输出量(42.81±4.91 ml vs. 37.06±4.75 ml)、射血分数(59.20%±5.41% vs. 44.56%±4.23%)、室壁增厚率（54.51%±8.60% vs. 43.36%±8.91%）、舒张期E峰 (54.85±6.35 cm/s vs. 43.14±4.81 cm/s)显著高于对照组(Plt;0.05)，左心室收缩期末容积(29.75±5.98 ml vs. 46.25±6.68 ml)、舒张期末容积(72.55±8.13 ml vs. 83.31±8.61 ml)显著低于对照组(Plt;0.05)。 结论 SIS作为支架移植MSCs有利于移植细胞存活，对陈旧性心肌梗死后心功能有明显的改善作用。