Objective To resolve the tough problem of how to observe the growing cells in an opaque vector. Methods The urethral epithelial cells from a young male New Zealand rabbit were inoculated, and were primarily cultured in vitro and subcultured for 3 passages. Then, the urethralepithelial cells were cultured in the collagen chitosan complex for 3, 7, 14 and 21 days. The cells were dyed with 6-carboxyfluorescein diacetateacetoxymethyl ester and propidium iodine, respectively. Then, Interactive Laser Cytometer was used to detect the growing cells. Results The urethral epithelial cells grew and proliferated very well in the collagen chitosan complex vector. After the urethral epithelial cells grew in the collagen-chitosan complex vector for 3 and 7 days, the fluorescent density amount of the surviving cells were(1.09±0.13)×10.8 and (2.04±0.13)×10.8, respectively. However, after 14and 21 days, the fluorescent density amount of the surviving cells was (0.55± 0.09)×10.8 and (0.47±0.03)×108, respectively. There was a significant difference when compared with the amount of the surviving cells at 3 and 7 days（P＜0.05）.Conclusion Using Interactive Laser Cytometer for measurement of the green and red fluorescent densities of different waves, the activity of the cultured urethral epithelial cells in vitro can be rapidlymeasured with the in situ quantitation method. This method solves a difficult problem of observing the growing cells in an opaque vector. The dynamic growing state of the engineering tissues can be observed.
目的探讨大隐静脉腔内激光+膝下经皮点状贯穿缝扎治疗大隐静脉曲张的临床疗效。方法回顾性分析我院2004年1月至2010年12月期间389例大隐静脉曲张患者采用腔内激光+膝下经皮点状贯穿缝扎术治疗的临床资料。结果手术全部成功完成，平均手术时间50 min。住院4～8 d，平均住院6 d。本组患者均获随访，随访时间为 1～36个月（平均18个月），所有患者均无深静脉血栓、深静脉损伤等手术并发症发生，无一例复发。迂曲、成团曲张静脉消失,溃疡愈合，色素沉着减轻或消失，下肢肿胀沉重感、酸困感消失。术后1个月彩超复查大隐静脉主干均全程闭塞，无血流信号，曲张的静脉均消失，膝下小腿部皮肤无条索状硬结及瘢痕。结论大隐静脉腔内激光结合膝下经皮点状贯穿缝扎术，使微创治疗大隐静脉曲张更加完善。
lfty white Leghorn hens were used,and 10 were randomly grouped as control,the other 40received the operation.A half of the profundus tendons of the second and third fore-toes of both sideswere cut.After the oporation,no Way immobiliZation was used.The oporated toes on one side wererandomly chosen as the treatment group,another side the oporated toes on the other side were served asthe control group. The toes having the injured tendons in the tratment group were irradiated for twentyminut...