Objective To study the expression of p16 and Rb gene in primary gallbladder carcinoma (PGC). MethodsFiftysix cases of PGC and 26 of gallbladder benign lesions were observed by immunohistochemistry (SP). ResultsThe positive rates of p16 and Rb protein in PGC were 46.3% and 71.4% respectively. No association of Rb expression with histological grade, histological type, invasion, metastasis and prognosis was found. There were close correlation between p16 expression and invasion, metastasis as well as prognosis. The inverse correlation of p16 and Rb gene expression in PGC was confirmed. Conclusion The loss of p16 or Rb gene protein is an important molecular event in PGC,and closely related to the block in the negative regulatory pathway of all cell cycle. The detection of p16 protein may be useful in evaluating biological characteristics and prognosis in PGC.
目的 探讨联合后-前胆囊三角入路在腹腔镜胆囊切除中的应用价值。方法 回顾性分析我院2007年1月至2010年1月期间经联合后-前胆囊三角入路解剖胆囊管及胆囊动脉行腹腔镜胆囊切除的240例患者的临床资料。结果 238例患者安全地完成腹腔镜胆囊切除,术中出血4例,均于镜下止血成功; 中转开腹2例。全组无胆管损伤,发生漏胆2例,经引流自愈。结论 联合后-前胆囊三角入路解剖胆囊管及胆囊动脉行腹腔镜胆囊切除是一种安全、容易掌握的手术方法。
目的探讨腹腔镜联合胆道镜治疗胆囊及胆总管结石的临床疗效。 方法回顾性分析2010年1月至2013年1月期间收治于笔者所在科室的96例胆囊及胆总管结石患者的临床资料,根据手术方式分为开腹组、腹腔镜联合胆道镜+T管引流术组和腹腔镜联合胆道镜+放置鼻胆管一期缝合组3组。对3组患者的手术时间、术中出血量、肛门排气时间、住院时间、残石率、复发率、并发症发生率、手术有效率及术后淀粉酶和肝功能指标水平进行对比分析。 结果微创手术组与开腹组比较,患者的出血量、肛门排气时间、住院时间、残石率、复发率及并发症发生率均减少、缩短或降低,同时手术的有效率提高(P<0.05);腹腔镜联合胆道镜+放置鼻胆管一期缝合组术后淀粉酶和肝功能指标的水平较其他2组明显降低(P<0.05)。 结论微创手术创伤小,残石率及复发率低,并发症少,患者术后恢复快,是一种安全有效的治疗方式,值得临床推广应用。
目的探讨腹腔镜胆囊切除术(LC)后并发出血的原因与正确的防治方法。方法对1992年10月至2005年2月我院进行的LC术后并发出血的37例患者的临床资料进行回顾性分析。结果胆囊动脉出血21例,胆囊床出血4例,网膜出血6例,trocar穿刺孔出血4例,不明出血部位2例。直接开腹手术7例,腹腔镜再次手术止血并获成功21例,腹腔镜止血失败中转开腹手术4例,非手术止血成功5例。36例顺利恢复出院,死亡1例。随访36例(其中失访1人),恢复良好。结论LC术后并发出血的原因多种多样,但多为胆囊动脉出血; 治疗以腹腔镜再次探查止血为主,但不能忽视非手术治疗的重要性。
Objective To explore the operative managements of ectopic gallbladder during laparoscopic cholecystectomy (LC).Methods Twenty one cases of ectopic gallbladder undergone LC in this hospital were analyzed regarding the perioperative management, principle, and technique of operation.Results There were 2 cases of situs transversus, 1 case with gallbladder under right posterior lobe of liver, 2 under left lateral lobe of liver and 16 in the liver. All 21 cases of ectopic gallbladder had undergone LC successfully, and no complications were found during and after operation. Conclusion Anatomic ectopia of gallbladder tosses a challenging problem to laparoscopic surgeon. It is safe for surgeons to recognise actual anatomical anomaly and to manage them appropriately.